A375.S2 - Cell Line (ID:50003)

HMS LINCS ID: 50003
Name: A375.S2
Alternative Names:
LINCS ID: LCL-1231
Alternative ID: CLO_0001583
Parent Cell Line:
Reference Source: ATCC CRL-1872
Organism: Homo sapiens
Organ: skin
Tissue:
Cell Type: epithelial
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:1909, malignant melanoma
Details of Disease:
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations:
Verification Reference Profile: DNA Profile (STR, source: ATCC): Amelogenin: X CSF1PO: 11,12 D13S317: 11,14 D16S539: 9 D5S818: 12 D7S820: 9 THO1: 8 TPOX: 8,10 vWA: 16,17
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Subculturing: Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution and let the culture sit at room temperature (or 37C) until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Relevant Citations:
Usage Note:
Date Publicly Available: 2012-04-25
Most Recent Update: 2016-07-12

Datasets:

HMS Dataset ID Dataset Title HMS Dataset Type
20003
Tang Mitosis/Apoptosis ver. II
Microscopy/Imaging

Batch Information:

HMS LINCS Batch ID Provider Provider Catalog ID Provider Batch ID
50003-1