S6 (pS235; pS236) / Alexa Fluor 647 - Antibody (ID:80005)

HMS LINCS ID: 80005
Name: S6 (pS235; pS236) / Alexa Fluor 647
Alternative Names: Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #4851
Clone Name: D57.2.2E
Resource Identification Initiative ID: AB_916160
LINCS ID:
Type: natural
Nominal Protein Target(s): S6 (pS235; pS236)
Nominal Protein Target HMS LINCS ID(s): 201298
Nominal Non-protein Target:
Organism of Nominal Target: Homo sapiens
Other Target Information: Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and 236. Species Reactivity: Human, Mouse, Rat, Monkey, Mink, S. cerevisiae. Species predicted to react based on 100% sequence homology: Chicken, Pig. (source: Cell Signaling Technology)
Other Human Protein Target HMS LINCS ID(s):
Immunogen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6. (source: Cell Signaling Technology)
Immunogen Sequence:
Fc Domain Source Species: rabbit
Clonality: monoclonal
Fc Domain Isotype: IgG
Source Organism for Production: rabbit
Production Details:
Conjugate or Label: Alexa Fluor 647
Labeling Details:
Relevant Citations:
Date Publicly Available: 2015-09-16
Most Recent Update: 2016-09-14

Datasets:

HMS Dataset ID Dataset Title HMS Dataset Type
20236
Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line
Microscopy/Imaging
20267
Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with EGF or four kinase inhibitors
Microscopy/Imaging
20303
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 1 of 6: Cytosolic fluorescent intensity measurements at 24 hr.
Microscopy/Imaging
20304
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 2 of 6: Nuclear fluorescent intensity measurements at 24 hr.
Microscopy/Imaging
20305
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 3 of 6: Cytosolic fluorescent intensity measurements at 48 hr.
Microscopy/Imaging
20306
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 4 of 6: Nuclear fluorescent intensity measurements at 48 hr.
Microscopy/Imaging
20307
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 5 of 6: Cytosolic fluorescent intensity measurements at 72 hr.
Microscopy/Imaging
20308
LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 6 of 6: Nuclear fluorescent intensity measurements at 72 hr.
Microscopy/Imaging

Batch Information:

HMS LINCS Batch ID Provider Provider Catalog ID Provider Batch ID
80005-1 Cell Signaling Technology 4851S 22